Alloantibody from a patient with severe von Willebrand disease inhibits von Willebrand factor-FVIII interaction
作者： J. Batlle , E. Lourés , P. Vila , M. C. Hernández , J. A. Méndez
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摘要: The aim of this study was to analyze the ability an alloantibody from a patient with severe von Willebrand disease (vWD) interfere vWF domain for FVIII, inhibit factor VIII (FVIII), and compare it rabbit polyclonal antibody. binding FVIII assayed by method previously described but using recombinant (r-FVIII, Kogenate), which contains no vWF, instead Hemofil M (HM). Rabbit or human antibodies towards (FVIII-Ab) were analyzed microtiter wells immobilized r-FVIII through monoclonal anti-FVIII antibody ELISA method. IgG plasma hemophilia A inhibitor used as positive control. Normal IgGs included negative controls. Human vWD anti-vWF reacted normal inhibiting its in dose-dependent manner, suggests that is specific. fraction, well nonspecific IgG, did not at all. assay immobilize alter interaction against showed partial inhibitory activity r-FVIII. inhibition reached plateau residual activity. FVIII-Ab detected preparations. In contrast, hemophiliac FVIII-AB. This behaves like heterologous antibodies, their seems be due steric hindrance mechanism, provided both have antibodies.
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