Novel thick filament protein of chicken pectoralis muscle: the 86 kd protein: II. Distribution and localization

摘要: Antibodies specific for the novel 86 kd protein purified from chicken pectoralis myofibrils stained by indirect immunofluorescence middle third of each half A-band isolated and myotubes. Pectoralis muscle protein, like C-protein, displayed a fibre-type distribution being restricted to fast twitch fibres absent in slow tonic heart fibres. This was demonstrated immunoblotting experiments with tissue extracts labelling cryosections. In primary cell cultures prepared embryonic breast muscle, C-protein myomesin were all detected post-mitotic myoblasts where fluorescence found cross-striated pattern along strands nascent myofibrils. Fluorescence due within myotubes no significant sarcoplasm evident. Glycerinated fibres, after incubation antibodies revealed nine distinctly labelled stripes, spaced about 43 nm apart. Simultaneous against showed co-localization seven stripes (stripes 5 11), protein. The two additional 3 4) anti-86 antibody continued towards M-band at same periodicity last stripe (stripe 5). Thus, partial different thick filament proteins is identity transverse positions 4 attributed part presence new