Extraction/Characterization of DNA

摘要: Abstract Some of the foundational procedures modern biology are extraction and characterization DNA from various cultures tissues. This chapter guides reader through key principles using some most commonly needed procedures, including preparation (1) plasmid bacterial cultures; (2) plant genomic DNA; (3) a commercial kit; (4) animal organic solvents; (5) fragments agarose gels. After each approach, quantity quality extracted is about same. As primary examples, we cover spectrophotometer as well fluorometric measurements. To verify integrity size DNA, gel electrophoresis remains used approach. Consequently, have included basic protocol that uses an alternative sodium borate buffer. The combination reliable with efficient steps allows downstream biological procedure to be performed successfully.