Nickel oxide (NiO) nanoparticles disturb physiology and induce cell death in the yeast Saccharomyces cerevisiae

摘要: The increasing use of nanoparticles (NPs) has spurred concerns about their toxic effects. This work aimed to assess the potential hazards nickel oxide (NiO) NPs using yeast Saccharomyces cerevisiae as a cell model. Yeast cells exposed for 6 h 100 mg/L NiO presented reduced metabolic activity (esterase and FUN-1 dye processing) enhanced accumulation reactive oxygen species. induced loss viability in dose-dependent manner. Study dissolution aqueous media, together with toxicological data, suggests that released by cannot explain all effects observed S. caused NPs. Transmission electron microscopy observations revealed were adsorbed onto surface but did not enter into cells. Isogenic mutants (cwp1∆ cwp2∆) increased wall porosity display susceptibility compared wild type strain. Our results suggest exert effect an indirect mechanism. contributes knowledge elucidation mechanisms action.