Preferential repression of the H subunit of ferritin by adenovirus E1A in NIH-3T3 mouse fibroblasts.

摘要: Ferritin is the major intracellular iron-storage protein in eucaryotic cells and plays a prominent role maintaining iron homeostasis. We observed that transfection of NIH-3T3 mouse fibroblasts with adenovirus E1A oncogene specifically repressed mRNA for one subunits ferritin, ferritin H. This occurred absence any effect on L subunit ferritin. The repression H was not general feature expression since H-ras did affect composition. Deletion conserved regions responsible immortalization transcriptional impaired ability to repress Immunoprecipitation transfectants demonstrated decrease H/L ratio at level also exhibited level. E1A-dependent inhibition chimeric gene containing promoter ligated chloramphenicol acetyltransferase reporter gene, but control genes which activity dependent promoters derived from SV40 or interleukin-3 gene. suggests may These results demonstrate modulates expression. They suggest alterations cellular metabolism be among diverse array responses induced by E1A.