Cellular expression, in-vitro and in-vivo confirmation of GAEC1 oncogenic properties in colon cancer

摘要: GAEC1 (Gene amplified in esophageal cancer 1) alterations have oncogenic properties oesophageal squamous cell carcinomas and frequent amplifications of the gene were noted colorectal adenocarcinomas. However, subcellular localization expression at protein level never been reported human cells. The present study aimed to investigate whether is differentially expressed different stages colon elucidate its underlying cellular molecular mechanism progression. We found differential mRNA pathological cells (SW480-Stage II, SW48-Stage III HCT116-Stage IV) when compared non-neoplastic (FHC cells) by immunocytochemistry, immunofluorescence, western blot analysis real-time polymerase chain reaction. was predominantly cytoplasm (SW480, SW48, HCT116) nucleus epithelial cells). transient knockdown using siRNA induced apoptosis SW480 SW48 cells, which associated with G2/M phase arrest decreased bcl-2 K-ras proteins increased p53. In addition, down-regulation significantly inhibited (p<0.05) proliferation, reduced migration capacity clonogenic potentiality (SW480 Furthermore, a xenotransplantation model showed that stable shRNA constructs fully suppressed xenograft tumour growth mice. Collectively, analysis, vitro vivo data indicated act as an oncogene