G Protein-Coupled Receptor 30 Localizes to the Endoplasmic Reticulum and Is Not Activated by Estradiol

摘要: The classical estrogen receptor (ER) mediates genomic as well rapid nongenomic estradiol responses. In case of responses, the ER acts a ligand-dependent transcription factor that regulates gene expression in target tissues. contrast, effects are initiated at plasma membrane and lead to activation cytoplasmic signal transduction pathways. Recently, an orphan G protein-coupled receptor, GPR30, has been claimed bind response estradiol. GPR30 therefore might mediate some effects. present study was performed clarify controversy about subcellular localization gain insight into vivo function this receptor. transiently transfected cells endogenously expressing we confirmed localized endoplasmic reticulum. However, using radioactive estradiol, observed only saturable, specific binding but not GP...