Preparation of cultured cells using high-pressure freezing and freeze substitution for subsequent 2D or 3D visualization in the transmission electron microscope.
作者： Nicole Doyle , Philippa C. Hawes
DOI: 10.1007/978-1-0716-0900-2_19
关键词:
摘要: … To prepare these samples for imaging in the TEM therefore requires extensive processing … of the material. Here we describe a method which aims to minimize preparation artifacts by …
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ucv.ve 参考文章(12)
Dimitri Vanhecke, Werner Graber, Daniel Studer, Close-to-native ultrastructural preservation by high pressure freezing. Methods in Cell Biology. ,vol. 88, pp. 151- 164 ,(2008) , 10.1016/S0091-679X(08)00409-3
Lilly Maneta-Peyret, Philippe Compére, Patrick Moreau, Gerhard Goffinet, Claude Cassagne, Immunocytochemistry of lipids: chemical fixatives have dramatic effects on the preservation of tissue lipids. Histochemical Journal. ,vol. 31, pp. 541- 547 ,(1999) , 10.1023/A:1003844107756
Müller M, Michel M, Studer D, High pressure freezing comes of age Scanning microscopy. ,vol. 3, pp. 253- 269 ,(1989)
K.L. McDONALD, R.I. WEBB, Freeze substitution in 3 hours or less. Journal of Microscopy. ,vol. 243, pp. 227- 233 ,(2011) , 10.1111/J.1365-2818.2011.03526.X
Kent L. McDonald, Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material Protoplasma. ,vol. 251, pp. 429- 448 ,(2014) , 10.1007/S00709-013-0575-Y
P Hawes, CL Netherton, M Mueller, T Wileman, P Monaghan, None, Rapid freeze-substitution preserves membranes in high-pressure frozen tissue culture cells. Journal of Microscopy. ,vol. 226, pp. 182- 189 ,(2007) , 10.1111/J.1365-2818.2007.01767.X
N. SARTORI, K. RICHTER, J. DUBOCHET, Vitrification depth can be increased more than 10‐fold by high‐pressure freezing Journal of Microscopy. ,vol. 172, pp. 55- 61 ,(1993) , 10.1111/J.1365-2818.1993.TB03393.X
Daniel Studer, Bruno M. Humbel, Matthias Chiquet, Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution. Histochemistry and Cell Biology. ,vol. 130, pp. 877- 889 ,(2008) , 10.1007/S00418-008-0500-1
D. Studer, W. Graber, A. Al-Amoudi, P. Eggli, A new approach for cryofixation by high‐pressure freezing Journal of Microscopy. ,vol. 203, pp. 285- 294 ,(2001) , 10.1046/J.1365-2818.2001.00919.X
D. Studer, M. Chiquet, E.B. Hunziker, Evidence for a distinct water-rich layer surrounding collagen fibrils in articular cartilage extracellular matrix. Journal of Structural Biology. ,vol. 117, pp. 81- 85 ,(1996) , 10.1006/JSBI.1996.0073