Isolation and characterization of an anther-specific gene, RA8, from rice (Oryza sativa L.).

摘要: An anther-specific cDNA clone of rice, RA8, was isolated from an anther library by differential screening. RNA blot analysis indicated that the RA8 transcript is present specifically in anthers and level increased as flowers matured, reaching highest mature flowers. The contains open reading frame 264 amino acid residues with a hydrophobic N-terminal region. deduced sequences did not show significant homology to any known sequences. Genomic DNA showed single-copy gene. A genomic corresponding its promoter region fused β-glucuronidase (GUS) Transgenic rice plants exhibited expression GUS reporter Histochemical active tapetum, endothecium, connective tissues anthers. Experiments gene starts when microspores are released tetrads, it reaches maximum at late vacuolated-pollen stage. may be useful for controlling cereal generating male-sterile plants.