Analysis of amplified DNAs from drug-resistant Leishmania by orthogonal-field-alternation gel electrophoresis. The effect of size and topology on mobility.
作者： R C Hightower , J B Bliska , N R Cozzarelli , D V Santi
DOI: 10.1016/S0021-9258(19)81709-X
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摘要: Abstract Amplified extrachromosomal DNAs from antifolate-resistant Leishmania are 30-75 kilobase (kb) supercoiled molecules that resolve on orthogonal-field-alternation gel electrophoresis (OFAGE) gels. These comigrate with smaller plasmids (7-8 kb), and their mobility is not a simple function of size. The properties the amplified were investigated to determine if an unusual structure accounts for observed by OFAGE; however, topological similar those standard Escherichia coli plasmids. migration series ranging in size 6 91 kb was analyzed OFAGE, triphasic pattern observed. mobilities between 20 60 increase size, whereas inversely proportional Like plasmids, large show pulse time-independent OFAGE. DNA accord plasmid markers. Therefore, it primarily Leishmania, rather than superhelical density or structure, results previously unexplained pattern.
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