Quantitative analysis of age-associated accumulation of mitochondrial DNA with deletion in human hearts.
作者： Satoru Sugiyama , Kazuki Hattori , Mika Hayakawa , Takayuki Ozawa
DOI: 10.1016/S0006-291X(05)81149-0
关键词:
摘要: Summary We have demonstrated that myocardial mitochondrial DNA (mtDNA) with a 7,436 base-pair deletion existed in all subjects were over 70 years old. Since each mitochondrion has two or three copies of its own DNA, quantitative analysis is required for the evaluation role mtDNA deletions age-related deterioration cardiac performance. For this purpose, kinetic polymerase chain reaction (PCR) method developed our laboratory was used investigation to determine human cadavers various ages. The population increased exponentially age [log f (% deleted mtDNA) = −3.136 + 0.0454 × age, r 0.95, P
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