Radiation resistance of the lung adenocarcinoma is related to the AKT-Onzin-POU5F1 axis.

摘要: Non-small cell lung carcinoma is the predominant type of cancer, and shows an easily developable tolerance to radiotherapy. Cancer stem cells are suggested be involved in resistance against therapies. Onzin might accumulated during process tumor overcoming radiation stress. To address relationship between Onzin, stemness resistance, we treated cancer bearing mice with radiaotherapy observed differences sensitive (RS) resistant (RR) tumors. Immunohistochemistry HE staining were used observe POU5F1 expression tissues. Quantitative realtime-PCR Western blot applied for tumors cells. In-vitro cellular viability was assessed by CCK8 methods derived The stably transfected A549 cell lines overexpressing generated through lentivirus transfection. After radiotherapy, those RR adenocarcinoma from them showed increased expression. Further, found upregulated stemness, indicated sphericity proliferation, as well Next, overexpressed A549 cells elevated expression, enhanced sphericity. Moreover, this could suppressed AKT inhibitor MK-2260. In vivo, not only higher formation capability growth, but also a significant radiation. Taken together, have Ectopic promotes functions, confers adenocarcinomas