Neuropilin 1 expression correlates with the Radio-resistance of human non-small-cell lung cancer cells

摘要: The purpose of this study was to determine the correlation between over-expression neuropilin 1 (NRP1) gene and growth, survival, radio-sensitivity non-small cell lung carcinoma (NSCLC) cells. 3-[4,5-dimethylthylthiazol-2-yl]-2,5 diphenyltetrazolium broide (MTT) colony assays were then performed effect NRP1 inhibition on in vitro growth NSCLC Annexin V-Fluorescein Isothiocyanate (FITC) apoptosis detection assay analyse enhancement Transwell invasion migration employed examine metastatic ability A549 cells post X-ray irradiation. In addition, Western blot carried out detect protein level VEGFR2, PI3K NF-κB. Finally, shNRP1 proliferation in vivo, a subcutaneous tumour formation in nude mice performed. Microvessel density tissues assessed by immunohistochemistry. stable transfected line (shNRP1-A549) showed significant reduction colony-forming not only in vitro, but also in vivo. Moreover, shRNA-mediated significantly enhanced both correlated with survival radio-resistance via VEGF-PI3K- NF-κB pathway, may be molecular therapeutic target for therapy or radio-sensitization NSCLC.