RNA interference (RNAi) inhibits growth of Plasmodium falciparum
作者： Louisa McRobert , Glenn A McConkey
DOI: 10.1016/S0166-6851(01)00429-7
关键词:
摘要: RNA interference (RNAi) causes degradation of targeted endogenous in many diverse organisms. Erythrocyte-infecting stages the malaria parasite Plasmodium falciparum were treated with double-stranded (dsRNA) encoding a segment gene dihydroorotate dehydrogenase (DHODH). DHODH is an enzyme pyrimidine biosynthesis, essential for growth. A decrease growth (P<0.0005) correlated levels mRNA. Control treatments single-stranded RNA, dsRNA circumsporozoite protein (a stage-specific not expressed asexual blood stage) and from related organism Toxoplasma gondii did inhibit As test RNAi assay, parasites chorismate synthase (CS), thought to be involved folate synthesis, examine requirement this Growth decreased (P<0.001) though less markedly than by DHODH. These results demonstrate utility assay assessing requirements products, their potential as chemotherapeutic targets.
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