Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas.

摘要: Seven psychrotolerant, Gram-negative bacterial strains, five dust- and airborne isolates (MA101bT, MA306a, MA405/90, MA-olkiT NW12T) two from the Antarctic (Ant 20 M3C203B-B), were subjected to a polyphasic characterization determine their taxonomic position. High 16S rDNA sequences similarities (99·3–100·0 %) demonstrated that they closely related each other. Phylogenetic evaluation of revealed are members genus Sphingomonas sensu stricto, encompassing separate branch within this genus. They shared 94·4–96·6 % sequence similarity with species All Sphingomonas-specific signature nucleotides also detected. The presence major ubiquinone Q-10, sym-homospermidine as predominant polyamine, Sphingomonadaceae-specific sphingoglycolipid in polar lipid patterns fatty acid profile containing C14 : 0 2-OH lacking 3-OH acids agreement identification these strains stricto. Results DNA–DNA hybridizations comparison protein indicated seven three distinct species. One is represented by MA101bT, MA306a second NW12T, Ant M3C203B-B third one strain, MA-olkiT. Their distinction at level was supported results biochemical partly riboprints genomic fingerprints. On basis results, novel proposed: aurantiaca sp. nov., consisting MA101bT (=DSM 14748T=LMG 21377T), MA405/90 14749=LMG 21378), faeni nov. 14747T=LMG 21379T) aerolata NW12T 14746T=LMG 21376T), (=ICMP 13599) (=SMCC M3C203B-B).