Advances in Reprogramming-Based Study of Neurologic Disorders

摘要: The technology to convert adult human non-neural cells into neural lineages, through induced pluripotent stem (iPSCs), somatic cell nuclear transfer, and direct lineage reprogramming or transdifferentiation has progressed tremendously in recent years. Reprogramming-based approaches aimed at manipulating cellular identity have enormous potential for disease modeling, high-throughput drug screening, therapy, personalized medicine. Human iPSC (hiPSC)-based models provided proof of principle evidence the validity this system. However, several challenges remain before patient-specific neurons produced by can provide reliable insights mechanisms be efficiently applied discovery transplantation therapy. This review will first discuss limitations currently available reprogramming-based methods faithfully reproducibly recapitulating pathology. Specifically, we address issues such as culture heterogeneity, interline inter-individual variability, two-dimensional differentiation paradigms. Second, assess progress future prospects neurologic modeling. includes three-dimensional advances technology, prescreening hiPSCs creating isogenic using gene editing.