ATP release from the human ureter on distension and P2X3 receptor expression on suburothelial sensory nerves

摘要: It is not clear how the increase in intraluminal pressure behind an obstructing ureteric calculus causes action potential frequency sensory nerves so pain messages are transmitted to brain. has been proposed that distension urothelial release of ATP, which activates purinoceptors on suburothelial nociceptive nerves. The purpose this study was determine whether human ureter results ATP and P2 receptor, P2X3, expressed ureter. Human segments were perfused with Krebs solution intermittently distended a range pressures. Samples perfusate collected throughout concentration ([ATP]) determined using luciferin-luciferase assay. Sections stained antibodies against P2X3 capsaicin receptors (TRPV1). [ATP] rose more than 10 times baseline levels after beyond threshold 25–30 cmH2O. Immunofluorescence studies consecutive frozen sections showed positively for receptors, no staining controls. These findings consistent hypothesis purinergic signalling involved mechanosensory transduction, leading nociception.