Soluble forms of NCAM and F3 neuronal cell adhesion molecules promote Schwann cell migration: identification of protein tyrosine phosphatases zeta/beta as the putative F3 receptors on Schwann cells.

摘要: Neural cell adhesion molecule (NCAM) and F3 are both axonal molecules which display homophilic or heterophilic (NCAM, F3) binding activities participate in bidirectional exchange of information between neurones glial cells. Engineered Fc chimeric fusion proteins that contain the extracellular part NCAM region human IgG1. Here, we investigated effect NCAM-Fc F3-Fc chimeras on Schwann (SC) migration. Binding sites were identified at surface cultured SCs by chimera coated fluorospheres. The functional was studied two different SC migration models. In first, is monitored specific time intervals inside a 1-mm gap produced monolayer culture SCs. second, from dorsal root ganglion explant migrate sciatic nerve cryosection. systems addition significantly increased extent this could be prevented corresponding anti-NCAM anti-F3 blocking antibodies. Furthermore, antiproteoglycan-type protein tyrosine phosphatase ζ/β (RPTPζ/β) antibodies presence RPTPζ/β enhancing soluble motility 95%. Sepharose beads precipitated lysates. Altogether these data point to putative receptor These results identify receptors as potential mediators signalling occurring axons cells during peripheral development regeneration.