Sulfatide Recognition by Colonization Factor Antigen CS6 from Enterotoxigenic Escherichia coli

摘要: The first step in the pathogenesis of enterotoxigenic Escherichia coli (ETEC) infections is adhesion bacterium to small intestinal epithelium. Adhesion ETEC mediated by a number antigenically distinct colonization factors, and among these, one most commonly detected non-fimbrial adhesin surface antigen 6 (CS6). potential carbohydrate recognition CS6 was investigated binding recombinant CS6-expressing E. purified protein large variant glycosphingolipids separated on thin-layer chromatograms. Thereby, highly specific coli, protein, sulfatide (SO3-3Galβ1Cer) obtained. bacteria inhibited dextran sulfate, but not dextran, heparin, galactose 4-sulfate or 6-sulfate. When using recombinantly expressed CssA CssB subunits complex, obtained with subunit, demonstrating that glycosphingolipid capacity resides within this subunit. CS6-binding present intestine species susceptible CS6-mediated infection, e.g. humans rabbits, lacking affected ETEC, mice. ability adhere target epithelium may thus contribute virulence.