Anchorage and lymphocyte function: extracellular matrix substrata control morphogenesis and interleukin production but have minor effects on DNA synthesis.

摘要: Contact with collagen and fibronectin substrata triggers disruption of aggregates activated lymphocytes, pseudopodia formation migration these lymphocytes onto the substrata. Monoclonal antibodies to alpha 4 5 chains beta 1-integrins inhibit cell substrate adhesion aggregate on A rat monoclonal antibody 1-integrin chain inhibits lymphocyte collagen. Two-dimensional (2D) three-dimensional (3D) have virtually same capacity abrogate aggregation. Fibronectin trigger initial phase(s) as effectively but later part process is relatively incomplete. Serum-coated plastic does not cause disruption. These results indicate that a specific event induced via surface receptors for extracellular matrix (ECM) components. major difference between 2D 3D seems be cells detach from former whereas latter infiltration dominates over detachment. Collagen are non-mitogenic they can modulate activation by allogeneic Con A. Thus, augment prolong such DNA synthesis, although slightly delay entry into S-phase decrease IL-2 production. substrata, particularly in form, also synthesis preactivated above magnitude serum-coated plastic. The nature substratum determines IL-1 Accordingly, spontaneous production mononuclear substantially lower than surfaces coated serum or BSA. However, factors which induce (e.g. LPS) more effective Abrogation aggregation, induction morphogenesis motile behaviour well control thus constitute effects ECM immune system. An additional minor influence cells, suggested present results, exerted modulation synthesis.