Anchorage and lymphocyte function: collagen and the maintenance of motile shape in T cells.

摘要: When cultured on a collagen matrix at density of 7 X 10(4) cells/cm2 for 48 hr, 80 +/- 10% unfractionated and 70 14% T-enriched human blood lymphocytes from eight healthy individuals developed motile morphology defined as the presence lamellar surface activity cytoplasmic flattening. During culture plastic 32 5% 38 6% same morphology. The was not rigid state but series oscillations in cell shape. conversion spherical into independent density. After preculture 'high' (1.5 10(6) cells/cm2), subsequent transfer to surface, 54 12% cells separate exhibited within 2 hr. These forms were, however, transient approximately 50% disappeared 12 Collagen augmented behaviour by two four times when fresh compared with glass or plastic. plastic, lost this prompt responsiveness contact. Thus, during ratio between percentage after respectively decreased values 4 immediately purification close 1 days. However, retransferred collagen, majority another 2-day period acquired measured potentiation shape substrate data suggest that variation T lymphocyte reflects labile property which is enhanced contact matrix.