Induction of motility and alteration of surface membrane polypeptides in lymphocytes by contact with autologous and allogeneic fibroblasts

摘要: Abstract Contact with cultured fibroblasts induced and maintained motile behavior in autologous allogeneic human lymphocytes. After 3 h of contact fibroblasts, 50 ± 19% the lymphocytes were after 24 corresponding figure was 49 18%. On a plastic surface number same individuals generally persisted below 15%. SDS-PAGE iodine-labeled indicated that but not for 3-h period caused appearance 300-kda band disappearance several bands lower molecular weight. During course T-lymphocyte activation by concanavalin A or cells on surface, forms did reach maximum (30 to 50% separate individuals) until 2 4 days culture. Thus, terms both rate development forms, fibroblast-dependent motility mechanism more effective than conventional lymphocyte blast transformation. Conditioned medium from induce provoke alteration membrane polypeptides as revealed iodination. Fibroblasts also triggered locomotion serum-free medium, their triggering effect enhanced markedly serum. The maintenance required protein synthesis. These data suggest during acquire locomotor capacity different