Molecular mechanism investigation of phenobarbital-induced serum cholesterol elevation in rat livers by microarray analysis.

摘要: Phenobarbital (PB) increases serum total cholesterol levels in rodents and humans. To investigate the underlying molecular mechanisms, we performed a microarray analysis on liver of rats treated repeatedly with 100 mg/kg PB, examined blood chemistry. The concentration non-esterified fatty acids was decreased from day 1 to 14 except for 7, that increased 4 14. ketone bodies triglycerides Transcript content glycolytic genes by PB treatments, while lipoprotein lipase continuously increased, suggesting notion repetitive treatments impaired glycolysis stimulated lipolysis liver. hypothesis using previously reported flux-balance model. increase mRNA malic enzyme after treatment agreed well model result, validity our hypothesis. findings also suggested there an abundance acetyl-CoA shortage products repeated treatments. Although ketogenesis would normally occur under such cellular conditions, it only weakly observed presumably owing decrease HMG-CoA synthase content. On other hand, several cholesterogenic slightly induced Thus, chemistry results cholesterogenesis rat livers, which may have contributed elevation concentration.