Utilization of a one-dimensional score for surveying chemical-induced changes in expression levels of multiple biomarker gene sets using a large-scale toxicogenomics database.
作者： Naoki KIYOSAWA , Kouji SHIWAKU , Mitsuhiro HIRODE , Ko OMURA , Takeki UEHARA
DOI: 10.2131/JTS.31.433
关键词:
摘要: A large-scale toxicogenomcis database has now been constructed in the Toxicogenomics Project Japan (TGP). To facilitate analytical procedures for such microarray data, we developed a simple one-dimensional score, named TGP1 which expresses trend of changes expression biomarker genes as whole. evaluate usefulness data rat liver and hepatocytes deposited TGP were scored three gene sets, i.e., carcinogenesis-related, PPARα-regulated glutathione depletion-related sets. The scoring system gave reasonable results, scores carcinogenesis-related high omeprazole-, chlorpromazine-, hexachlorobenzene-, sulfasalazine- Wy-14,643-treated livers, that clofibrate-, Wy-14,643-, gemfibrozil-, benzbromarone- aspirin-treated livers well hepatocytes, deficiency-related bromobenzene-, acetaminophen- coumarin-treated liver. We concluded score is useful surveying multiple sets toxicogenomics database, would reduce time doing conventional multivariate statistical analysis. In addition, can be applied to screening compatible between like will allow us develop an appropriate vitro drug safety assessment vivo.
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