Evaluation of DNA microarray results in the Toxicogenomics Project (TGP) consortium in Japan.
作者： Nakatsu Noriyuki , Yoshinobu Igarashi , Atsushi Ono , Hiroshi Yamada , Yasuo Ohno
DOI: 10.2131/JTS.37.791
关键词:
摘要: An important technology used in toxicogenomic drug discovery research is the microarray, which enables researchers to simultaneously analyze expression of a large number genes. To build database and data analysis system for use assessing safety drugs candidates, 2002 we conducted 5-year collaborative study Toxicogenomics Project (TGP1) Japan. Experimental generated by such studies must be validated different laboratories robust accurate analysis. For this purpose, intra- inter-laboratory validation with participating companies second (TGP2). Gene liver rats treated acetaminophen (APAP) was independently examined using Affymetrix GeneChip microarrays. The reproducibility evaluated hierarchical clustering toxicogenomics results were highly reproducible, indicating that gene our TGP1 project reliable compatible laboratories.
japanlinkcenter.org
elsevier.com参考文章(25)
Jun Kanno, Ken-ichi Aisaki, Katsuhide Igarashi, Noriyuki Nakatsu, Atsushi Ono, Yukio Kodama, Taku Nagao, "Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays BMC Genomics. ,vol. 7, pp. 64- 64 ,(2006) , 10.1186/1471-2164-7-64
Maureen A Sartor, Craig R Tomlinson, Scott C Wesselkamper, Siva Sivaganesan, George D Leikauf, Mario Medvedovic, Intensity-based hierarchical Bayes method improves testing for differentially expressed genes in microarray experiments BMC Bioinformatics. ,vol. 7, pp. 538- 538 ,(2006) , 10.1186/1471-2105-7-538
Peng Liang, MAQC papers over the cracks. Nature Biotechnology. ,vol. 25, pp. 27- 28 ,(2007) , 10.1038/NBT0107-27
Naoki KIYOSAWA, Kouji SHIWAKU, Mitsuhiro HIRODE, Ko OMURA, Takeki UEHARA, Toshinobu SHIMIZU, Yumiko MIZUKAWA, Toshikazu MIYAGISHIMA, Atsushi ONO, Taku NAGAO, Tetsuro URUSHIDANI, Utilization of a one-dimensional score for surveying chemical-induced changes in expression levels of multiple biomarker gene sets using a large-scale toxicogenomics database. Journal of Toxicological Sciences. ,vol. 31, pp. 433- 448 ,(2006) , 10.2131/JTS.31.433
Mitsuhiro Hirode, Ko Omura, Naoki Kiyosawa, Takeki Uehara, Toshinobu Shimuzu, Atsushi Ono, Toshikazu Miyagishima, Taku Nagao, Yasuo Ohno, Tetsuro Urushidani, Gene expression profiling in rat liver treated with various hepatotoxic-compounds inducing coagulopathy. Journal of Toxicological Sciences. ,vol. 34, pp. 281- 293 ,(2009) , 10.2131/JTS.34.281
Ko OMURA, Naoki KIYOSAWA, Takeki UEHARA, Mitsuhiro HIRODE, Toshinobu SHIMIZU, Toshikazu MIYAGISHIMA, Atsushi ONO, Taku NAGAO, Tetsuro URUSHIDANI, Gene expression profiling of rat liver treated with serum triglyceride-decreasing compounds. Journal of Toxicological Sciences. ,vol. 32, pp. 387- 399 ,(2007) , 10.2131/JTS.32.387
Takeki Uehara, Naoki Kiyosawa, Mitsuhiro Hirode, Ko Omura, Toshinobu Shimizu, Atsushi Ono, Yumiko Mizukawa, Toshikazu Miyagishima, Taku Nagao, Tetsuro Urushidani, Gene expression profiling of methapyrilene-induced hepatotoxicity in rat. Journal of Toxicological Sciences. ,vol. 33, pp. 37- 50 ,(2008) , 10.2131/JTS.33.37
Koji Kadota, Yuji Nakai, Kentaro Shimizu, None, A weighted average difference method for detecting differentially expressed genes from microarray data Algorithms for Molecular Biology. ,vol. 3, pp. 8- 8 ,(2008) , 10.1186/1748-7188-3-8
Naoki KIYOSAWA, Takeki UEHARA, Weihua GAO, Ko OMURA, Mitsuhiro HIRODE, Toshinobu SHIMIZU, Yumiko MIZUKAWA, Atsushi ONO, Toshikazu MIYAGISHIMA, Taku NAGAO, Tetsuro URUSHIDANI, IDENTIFICATION OF GLUTATHIONE DEPLETION-RESPONSIVE GENES USING PHORONE-TREATED RAT LIVER Journal of Toxicological Sciences. ,vol. 32, pp. 469- 486 ,(2007) , 10.2131/JTS.32.469
M Hirode, A Horinouchi, T Uehara, A Ono, T Miyagishima, H Yamada, T Nagao, Y Ohno, T Urushidani, Gene expression profiling in rat liver treated with compounds inducing elevation of bilirubin. Human & Experimental Toxicology. ,vol. 28, pp. 231- 244 ,(2009) , 10.1177/0960327109104528