摘要: Understanding how molecular motors generate force and move microtubules in mitosis is essential to understanding the physical mechanism of cell division. Recent measurements have shown that one mitotic kinesin superfamily member, Eg5, mechanically processive capable crosslinking sliding vitro. In this review, we highlight recent work explores Eg5 functions under load, with an emphasis on nanomechanical properties single enzymes.
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William M. Saxton, Douglas G. Cole, Jonathan M. Scholey, Kathy B. Sheehan, A “Slow” Homotetrameric Kinesin-related Motor Protein Purified from Drosophila Embryos Journal of Biological Chemistry. ,vol. 269, pp. 22913- 22916 ,(1994) , 10.1016/S0021-9258(17)31593-4
Taro Q.P. Uyeda, Stephen J. Kron, James A. Spudich, Myosin step size. Estimation from slow sliding movement of actin over low densities of heavy meromyosin. Journal of Molecular Biology. ,vol. 214, pp. 699- 710 ,(1990) , 10.1016/0022-2836(90)90287-V
Michael J. deCastro, Regis M. Fondecave, Leigh A. Clarke, Christoph F. Schmidt, Russell J. Stewart, Working strokes by single molecules of the kinesin-related microtubule motor ncd Nature Cell Biology. ,vol. 2, pp. 724- 729 ,(2000) , 10.1038/35036357
Torsten Wittmann, Anthony Hyman, Arshad Desai, The spindle: a dynamic assembly of microtubules and motors. Nature Cell Biology. ,vol. 3, ,(2001) , 10.1038/35050669
R Le Guellec, J Paris, A Couturier, C Roghi, M Philippe, Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein. Molecular and Cellular Biology. ,vol. 11, pp. 3395- 3398 ,(1991) , 10.1128/MCB.11.6.3395
Masahide Kikkawa, Elena P. Sablin, Yasushi Okada, Hiroaki Yajima, Robert J. Fletterick, Nobutaka Hirokawa, Switch-based Mechanism of Kinesin Motors Nature. ,vol. 411, pp. 439- 445 ,(2001) , 10.1038/35078000
Paul Maddox, Arshad Desai, Karen Oegema, Timothy J. Mitchison, E.D. Salmon, Poleward Microtubule Flux Is a Major Component of Spindle Dynamics and Anaphase A in Mitotic Drosophila Embryos Current Biology. ,vol. 12, pp. 1670- 1674 ,(2002) , 10.1016/S0960-9822(02)01183-1
Steven M. Block, Nanometres and piconewtons: the macromolecular mechanics of kinesin. Trends in Cell Biology. ,vol. 5, pp. 169- 175 ,(1995) , 10.1016/S0962-8924(00)88982-5
Ahmet Yildiz, Michio Tomishige, Ronald D Vale, Paul R Selvin, Kinesin Walks Hand-Over-Hand Science. ,vol. 303, pp. 676- 678 ,(2004) , 10.1126/SCIENCE.1093753
Michael J. deCastro, Chih-Hu Ho, Russell J. Stewart, Motility of dimeric ncd on a metal-chelating surfactant: evidence that ncd is not processive. Biochemistry. ,vol. 38, pp. 5076- 5081 ,(1999) , 10.1021/BI9829175