The Homotetrameric Kinesin-5 KLP61F Preferentially Crosslinks Microtubules into Antiparallel Orientations

摘要: The segregation of genetic material during mitosis is coordinated by the mitotic spindle, whose action depends upon polarity patterns its microtubules (MTs). Homotetrameric kinesin-5 motors can crosslink and slide adjacent spindle MTs, but it unknown whether they or other contribute to establishing these MT patterns. Here, we explored Drosophila embryo KLP61F, which plausibly crosslinks both parallel antiparallel displays a preference for orientation. In motility assays, KLP61F was observed as predicted. Remarkably, displayed 3-fold higher crosslinking MTs in This presence ADP ATP plus AMPPNP, not AMPPNP alone, induces instantaneous rigor binding. Also, purified motorless tetramer containing C-terminal tail domains an orientation preference, confirming that motor activity required. results suggest that, morphogenesis KLP61F's sliding activities could facilitate gradual accumulation within interpolar at equator, where generate force drive poleward flux pole-pole separation.