Partition of proteins in the aqueous guar/dextran two-phase system
作者： F Simonet , C Garnier , J.-L Doublier
DOI: 10.1016/S0268-005X(00)00041-2
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摘要: Abstract The partition of proteins (bovine serum albumin, β-lactoglobulin, micellar casein) has been examined in the guar/dextran aqueous two-phase system. influence parameters like pH, ionic strength (NaCl), and polymer concentration investigated while protein was increased up to 2% w/w. For globular β-lactoglobulin) general trends partitioning established literature with other systems polyethyleneoxide/dextran were nearly verified. dextran-rich bottom phase found be slightly more concentrated than guar-enriched top one. However, casein displayed a different behavior, giving uneven separation phenomena at concentrations above 0.5% This difference attributed larger size particle. Meanwhile, modifications diagram have investigated. It shown that addition or w/w did not affect thermodynamic features this
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