Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli

摘要: Glycolate is a valuable chemical with extensive applications in many different fields. The traditional methods to synthesize glycolate are quite expensive and toxic. So, the biotechnological production of from sustainable feedstocks interest for its potential economic environmental advantages. d-Xylose second most abundant sugar nature accounts 18–30% lignocellulose. New routes conversion xylose were explored. Overexpression aceA ghrA deletion aceB Escherichia coli examined xylose, but was initially ineffective. Then, new route established E. by introducing NAD+-dependent dehydrogenase (xdh) xylonolactonase (xylC) Caulobacter crescentus. constructed engineered strain Q2562 produced 28.82 ± 0.56 g/L 0.60 ± 0.01 g/L/h productivity 0.38 ± 0.07 g/g yield. However, 27.18 ± 2.13 g/L acetate accumulated after fermentation. Deletions iclR ackA used overcome excretion. An knockout resulted about 66% decrease formation. final Q2742 43.60 ± 1.22 g/L glycolate, 0.91 ± 0.02 g/L/h 0.46 ± 0.03 g/g A established, an this explored pathway. engineering showed highest reported date. This research opened up prospect bio-refinery alternative choice industrial glycolate.