Metabolic engineering of Escherichia coli to improve recombinant protein production.

摘要: Escherichia coli is one of the most widely used strains for recombinant protein production. However, obstacles also exist in both academic researches and industrial applications, such as metabolic burden, carbon source waste, cells' physiological deterioration. This article reviews recent approaches improving production engineering, including workhorse selection, stress factor application, flux regulation. Selecting a suitable host first key point In general, it all depends on characteristics target proteins. It will be triggered cells deterioration when medium significantly different from cell's natural environment. Coexpression factors can help proteins to fold into their native conformation. Carbon regulation direct approach redirecting more toward desirable pathways products. some undesirable consequences are usually found glucose transport inhibition, cell growth retardation, useless metabolite accumulation. More efficient regulators platform factories should explored meet variety demands.