Increased recombinant protein production in Escherichia coli strains with overexpressed water-forming NADH oxidase and a deleted ArcA regulatory protein.
作者： G.N. Vemuri , M.A. Eiteman , E. Altman
DOI: 10.1002/BIT.20853
关键词:
摘要: Glycolytic flux is increased and acetate production reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with deletion arcA gene, which encodes ArcA regulatory protein. In this study, we examined overproduction a model recombinant protein strains E. expressing NOX or without an mutation. The presence absence about 50% beta-galactosidase 10-20%. strain eliminated entirely batch fermentations resulted 120% increase production.
sci-hub.se 参考文章(31)
T. Paalme, R. Elken, A. Kahru, K. Vanatalu, R. Vilu, The growth rate control in Escherichia coli at near to maximum growth rates: the A-stat approach Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 71, pp. 217- 230 ,(1997) , 10.1023/A:1000198404007
K B Andersen, K von Meyenburg, Are growth rates of Escherichia coli in batch cultures limited by respiration Journal of Bacteriology. ,vol. 144, pp. 114- 123 ,(1980) , 10.1128/JB.144.1.114-123.1980
A.V. Fowler, I. Zabin, Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence. Journal of Biological Chemistry. ,vol. 253, pp. 5521- 5525 ,(1978) , 10.1016/S0021-9258(17)30405-2
G W Luli, W R Strohl, Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Applied and Environmental Microbiology. ,vol. 56, pp. 1004- 1011 ,(1990) , 10.1128/AEM.56.4.1004-1011.1990
G L Kleman, W R Strohl, Acetate metabolism by Escherichia coli in high-cell-density fermentation. Applied and Environmental Microbiology. ,vol. 60, pp. 3952- 3958 ,(1994) , 10.1128/AEM.60.11.3952-3958.1994
R. A. Majewski, M. M. Domach, Simple constrained-optimization view of acetate overflow in E. coli Biotechnology and Bioengineering. ,vol. 35, pp. 732- 738 ,(1990) , 10.1002/BIT.260350711
Wayne A. Johnston, Meredith Stewart, Peter Lee, Michael J. Cooney, Tracking the acetate threshold using DO‐transient control during medium and high cell density cultivation of recombinant Escherichia coli in complex media Biotechnology and Bioengineering. ,vol. 84, pp. 314- 323 ,(2003) , 10.1002/BIT.10772
R. R. Gokarn, J. D. Evans, J. R. Walker, S. A. Martin, M. A. Eiteman, E. Altman, The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli. Applied Microbiology and Biotechnology. ,vol. 56, pp. 188- 195 ,(2001) , 10.1007/S002530100661
M. Åkesson, P. Hagander, J. P. Axelsson, Avoiding acetate accumulation in Escherichia coli cultures using feedback control of glucose feeding Biotechnology and Bioengineering. ,vol. 73, pp. 223- 230 ,(2001) , 10.1002/BIT.1054
M.A. Eiteman, M.J. Chastain, Optimization of the ion-exchange analysis of organic acids from fermentation Analytica Chimica Acta. ,vol. 338, pp. 69- 75 ,(1997) , 10.1016/S0003-2670(96)00426-6