Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli.
作者： David J. Wurm , Julian Quehenberger , Julia Mildner , Britta Eggenreich , Christoph Slouka
DOI: 10.1007/S00253-017-8641-6
关键词:
摘要: … of such structures actually allows a comparably mild treatment during IB processing to maintain the already correctly folded secondary structures and thus increase the refolding yield. …
springer.com
paperity.org参考文章(52)
Pietro Gatti-Lafranconi, Antonino Natalello, Diletta Ami, Silvia Maria Doglia, Marina Lotti, Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology FEBS Journal. ,vol. 278, pp. 2408- 2418 ,(2011) , 10.1111/J.1742-4658.2011.08163.X
Min Liu, Xinjun Feng, Yamei Ding, Guang Zhao, Huizhou Liu, Mo Xian, Metabolic engineering of Escherichia coli to improve recombinant protein production. Applied Microbiology and Biotechnology. ,vol. 99, pp. 10367- 10377 ,(2015) , 10.1007/S00253-015-6955-9
F A Marston, The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochemical Journal. ,vol. 240, pp. 1- 12 ,(1986) , 10.1042/BJ2400001
L Strandberg, S O Enfors, Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli. Applied and Environmental Microbiology. ,vol. 57, pp. 1669- 1674 ,(1991) , 10.1128/AEM.57.6.1669-1674.1991
E. C. FRIEDBERG, The Molecular Biology of Nucleotide Excision Repair of DNA: Recent Progress Journal of Cell Science. ,vol. 6, pp. 1- 23 ,(1987) , 10.1242/JCS.1984.SUPPLEMENT_6.1
Thomas Baumgarten, Dirk Jan Slotboom, Jan-Willem de Gier, Anna Hjelm, Zhe Zhang, Grietje Kuipers, Łukasz Niemiec, High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG. Microbial Cell Factories. ,vol. 14, pp. 142- 142 ,(2015) , 10.1186/S12934-015-0328-Z
A. Kremling, J. Geiselmann, D. Ropers, H. de Jong, Understanding carbon catabolite repression in Escherichia coli using quantitative models. Trends in Microbiology. ,vol. 23, pp. 99- 109 ,(2015) , 10.1016/J.TIM.2014.11.002
Maria K. Eiberle, Alois Jungbauer, Technical refolding of proteins: Do we have freedom to operate? Biotechnology Journal. ,vol. 5, pp. 547- 559 ,(2010) , 10.1002/BIOT.201000001
Špela Peternel, Radovan Komel, Active Protein Aggregates Produced in Escherichia coli International Journal of Molecular Sciences. ,vol. 12, pp. 8275- 8287 ,(2011) , 10.3390/IJMS12118275
César Díez-Gil, Sven Krabbenborg, Elena García-Fruitós, Esther Vazquez, Escarlata Rodríguez-Carmona, Imma Ratera, Nora Ventosa, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Neus Ferrer-Miralles, Antonio Villaverde, Jaume Veciana, The nanoscale properties of bacterial inclusion bodies and their effect on mammalian cell proliferation. Biomaterials. ,vol. 31, pp. 5805- 5812 ,(2010) , 10.1016/J.BIOMATERIALS.2010.04.008