Fibrin structural and diffusional analysis suggests that fibers are permeable to solute transport

摘要: Fibrin hydrogels are promising carrier materials in tissue engineering. They biocompatible and easy to prepare, they can bind growth factors be prepared from a patient’s own blood. While fibrin structure mechanics have been extensively studied, not much is known about the relation between diffusivity of solutes within network. This particularly relevant for with size similar that factors. A novel methodological approach has used this study retrieve quantitative structural characteristics hydrogels, by combining two complementary techniques, namely confocal fluorescence microscopy fiber extraction algorithm turbidity measurements. Bulk rheological measurements were conducted determine impact hydrogel on mechanical properties. From these it concluded variations large response (up orders magnitude difference storage modulus) but only moderate influence dextran 25% difference). By analyzing means Ogston diffusion model we further provide evidence individual fibers semi-permeable solute transport, depending average distance protofibrils. important reducing mass transport limitations, modulating fibrinolysis factor binding, which all