A causal relation between bioluminescence and oxygen to quantify the cell niche.

摘要: Bioluminescence imaging assays have become a widely integrated technique to quantify effectiveness of cell-based therapies by monitoring fate and survival transplanted cells. To date these are still largely qualitative often erroneous due the complexity dynamics local micro-environments (niches) in which cells reside. Here, we report, using combined experimental computational approach, on oxygen that besides being critical niche component responsible for cellular energy metabolism cell-fate commitment, also serves primary role regulating bioluminescent light kinetics. We demonstrate potential an dependent Michaelis-Menten relation quantifying intrinsic bioluminescence intensities resolving cell-associated gradients from is emitted three-dimensional (3D) cell-seeded hydrogels. Furthermore, data indicate strong causal concentration with intensities. Altogether our approach demonstrates importance evolve towards quantitative holds great future microscale measurement tension easily accessible manner.