Production of human or humanized antibodies in mice

摘要: Mice are widely available laboratory animals that can easily be used for the production of antibodies against a broad range antigens, using well-defined immunization protocols. Such an approach allows optimal in vivo affinity maturation humoral response. In addition, high-affinity arising this context readily further characterized and produced as monoclonals after immortalizing selecting specific antibody-producing cells through hybridoma derivation. Using such conventional strategies combined with mice either genetically engineered to carry humanized immunoglobulin (Ig) genes or engrafted human immune system, it is thus easy obtain immortalize clones produce fully Ig associating variable (V) domains selected antigen specificities customized human-like constant regions, defined effector functions. some instances, where there need functional assays single antibody known specificity, might interest transiently express gene by transfer. This rapid assay. More commonly, diversified repertoire producing molecules mouse B cell lineage from strains transgene which human, humanized, chimeric origin. After response, will lead secretion optimized binding sites, associated desired domains. chapter focuses on two simple methods: (1) (2) hydrodynamics-based transfection.