Cre-loxP-mediated gene replacement: a mouse strain producing humanized antibodies

摘要: Abstract Background: The bacteriophage-derived Cre– loxP recombination system operates efficiently in mammalian cells. This is particularly useful gene-targeting experiments the mouse, and has already been used to generate ‘clean' deletions of target genes germ line, as well inactivate a conditional manner (based on regulated expression Cre recombinase). In principle, -mediated should also allow gene replacement, thus introduction virtually any kind mutation into genome. Results We system, mouse embryonic stem cells, replace Cγ 1, which encodes constant region heavy chain IgG1 antibodies, with its human counterpart. was transmitted through resulting mutant mice were crossed expressing κ light chains human, instead region. Mice homozygous for both mutations produce humanized, -chain-bearing antibodies at same level efficiency wild-type murine antibodies. These animals enable ex vivo production chimeric monoclonal specific antigen can respond. Conclusion replacement simple efficient general method targeted mutagenesis mouse.