Production of hybrid double- or triple-layered virus-like particles of group A and C rotaviruses using a baculovirus expression system.
作者： Yunjeong Kim , Kyeong-Ok Chang , Won-Yong Kim , Linda J Saif , None
关键词:
摘要: Abstract Dual infections by group A and C rotaviruses have been reported, but no reassortants between described. The VP6 major inner capsid protein of shares common antigens detected monoclonal antibodies also 40–43% amino acid identity. Coinfection Spodoptera frugiperda (Sf9) insect cells with different combinations the recombinant baculoviruses encoding either {RF VP2 (A-VP2), IND (A-VP6), VP7 (A-VP7[IND]), 2292B (A-VP7[2292B])} or [Shintoku (C-VP6) (C-VP7)] bovine rotavirus proteins produced hybrid A/C triple-layered VP2/6/7 virus-like particles (TLPs) composed A-VP2/C-VP6/C-VP7, A-VP2/C-VP6/A-VP7(IND), A-VP2/C-VP6/A-VP7(2292B), A-VP2/A-VP6/C-VP7. To our knowledge, this is first report that can support attachment heterologous, antigenically distinct (G6, G10, 2292B) outer to produce TLPs in vitro.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(45)
L. J. Saif, B. Jiang, Nongroup A rotaviruses of humans and animals. Current Topics in Microbiology and Immunology. ,vol. 185, pp. 339- 371 ,(1994) , 10.1007/978-3-642-78256-5_11
Paul A. Offit, Rotaviruses: immunological determinants of protection against infection and disease. Advances in Virus Research. ,vol. 44, pp. 161- 202 ,(1994) , 10.1016/S0065-3527(08)60329-2
Robert F. Ramig, Richard L. Ward, Genomic segment reassortment in rotaviruses and other reoviridae. Advances in Virus Research. ,vol. 39, pp. 163- 207 ,(1991) , 10.1016/S0065-3527(08)60795-2
H Tsunemitsu, B Jiang, Y Yamashita, M Oseto, H Ushijima, L J Saif, Evidence of serologic diversity within group C rotaviruses. Journal of Clinical Microbiology. ,vol. 30, pp. 3009- 3012 ,(1992) , 10.1128/JCM.30.11.3009-3012.1992
Albert Z. Kapikian, Viral infections of the gastrointestinal tract Marcel Dekker. ,(1994) , 10.1201/9781498710541
S E Crawford, M Labbé, J Cohen, M H Burroughs, Y J Zhou, M K Estes, Characterization of virus-like particles produced by the expression of rotavirus capsid proteins in insect cells. Journal of Virology. ,vol. 68, pp. 5945- 5952 ,(1994) , 10.1128/JVI.68.9.5945-5952.1994
M Labbé, A Charpilienne, S E Crawford, M K Estes, J Cohen, Expression of rotavirus VP2 produces empty corelike particles. Journal of Virology. ,vol. 65, pp. 2946- 2952 ,(1991) , 10.1128/JVI.65.6.2946-2952.1991
G Tosser, M Labbé, M Brémont, J Cohen, Expression of the major capsid protein VP6 of group C rotavirus and synthesis of chimeric single-shelled particles by using recombinant baculoviruses. Journal of Virology. ,vol. 66, pp. 5825- 5831 ,(1992) , 10.1128/JVI.66.10.5825-5831.1992
Richard L Ward, Osamu Nakagomi, Douglas R Knowlton, Monica M McNeal, Toyoko Nakagomi, Nurul Huda, John D Clemens, David A Sack, None, Formation and selection of intergenogroup reassortants during cell culture adaptation of rotaviruses from dually infected subjects. Journal of Virology. ,vol. 65, pp. 2699- 2701 ,(1991) , 10.1128/JVI.65.5.2699-2701.1991
B I Rosen, A V Parwani, M Gorziglia, G Larralde, L J Saif, Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. Journal of Clinical Microbiology. ,vol. 30, pp. 2644- 2652 ,(1992) , 10.1128/JCM.30.10.2644-2652.1992