Method and device for fluorescent measurement of samples

摘要: The invention provides a method of fluorescent measurement sample and corresponding measuring device. includes impinging an incident beam having excitation wavelength focus diameter on the to yield laser spot that defines volume for fluorescence. then displacing relative in three dimensional space defined by define individual interest. further comprises translating across depth direction purpose defining profile based thickness carrier detecting one or more emitted fluorescence signals from direction, determining at least microvolume interest obtaining concentrated signal.