Method for accurate counting of probe spots in cell nuclei

摘要: A method for the accurate counting of DNA probe spots in cell nuclei wherein anomalies caused by a two dimensional measurement three sample are eliminated from evaluation. which counted means Fluorescent In Situ Hybridization (FISH) include cells different countoured overlaid or detected as being adjacent one another with resultant erroneous diagnostic results such cancer detection prognosis. gating fluorescent value is determined clusters fluorescence regions non-anormalous values plotting peak flouorescent against area. The loci non-anomalous cluster specifically definable whereby deviate value, discounted preparation histograms other measurements.