Method and apparatus for measuring multiple optical properties of biological specimens

摘要: The invention relates to an apparatus and methods for generating multiparameter optical data that characterize a population of cells. includes the steps scanning cell with beam produce sets digital samples, each sample set representing interactions from specific location within population; storing data, e.g., in computer memory; locating population, by comparing preselected threshold value; defining neighborhood around located cell; estimating background level based upon corresponding locations outside neighborhood; correcting samples estimated generate data. further correction calibration as well sampling features enable precise estimates multiple cellular constituents or other properties at high rates speed.