Neuropeptide Y in hypothalamic paraventricular nucleus: a center coordinating energy metabolism

摘要: Intracerebroventricular injection of neuropeptide Y (NPY) has two effects on energy metabolism in addition to increased feeding: decreased brown fat thermogenesis and white lipoprotein lipase (LPL) enzymatic activity. We hypothesized that the paraventricular nucleus (PVN) hypothalamus is controlling neural site for these responses. further NPY stimulation at PVN would reduce gene expression critical thermogenic protein, uncoupling protein (UCP), increase key storage enzyme, LPL. In first experiment, three groups rats received injections every 6 h 24 (5 total) into PVN:1) (1 micrograms/1 microliters injection) ad libitum food; 2) food restricted control intake; 3) saline microliter) food. Both NPY-treated showed significant reductions (P < 0.05) UCP mRNA levels marked LPL relative controls. second four seven had injected PVN:0 (vehicle control); 0.1 microgram; 0.5 1 microgram. Injections were made h. There was a dose-related reduction produced by treatment. treatment mRNA, but smooth dosing effect not evident. The observation can coordinate more than one component when considered with many reports responsiveness activity arcuate nucleus-PVN circuit perturbations balance such as fasting feeding, diabetes, genetic obesity.(ABSTRACT TRUNCATED AT 250 WORDS)