Evaluation of experimentally induced Fusobacterium necrophorum infections in mice.

摘要: Two strains of mice, Swiss Webster and DBA/2Cr, were injected intraperitoneally or intravenously with varying dosages Fusobacterium necrophorum. The ability to eliminate the infection was assessed by quantitative enumeration organisms present in blood, liver, spleen, Three- 4-week-old DBA/2Cr mice highly resistant both routes injection. intraperitoneal injection older failed demonstrate a dose-effect relationship whereas an intravenous as few 10(4) cells F. necrophorum produced progressively necrotic leg abscesses, apparently involving lymphonodus ischiadicus which filters site Mortality increased sensitization previous sublethal Also, ethanol-killed cell vaccine delayed onset lethal infection, repeated live injections provided nonspecific protection since vaccinated growth medium equally protected. development abscesses after visibly demonstrated pathogenicity may provide suitable model for evaluation vaccines effectiveness antibiotics.