Immunogenicity and protective effects of truncated recombinant leukotoxin proteins of Fusobacterium necrophorum in mice.

摘要: Fusobacterium necrophorum, a gram-negative, anaerobic and rod-shaped bacterium, is generally an opportunistic pathogen causes wide variety of necrotic infections in animals humans. Leukotoxin, secreted protein, major virulence factor. The gene encoding the leukotoxin (lktA) F. necrophorum has been cloned, sequenced expressed Escherichia coli. Because low expression levels, problems associated with purifying full-length recombinant physical instability five overlapping truncations were constructed. polypeptides (BSBSE, SX, GAS, SH, FINAL) E. coli purified by nickel-affinity chromatography. objectives to investigate effectiveness truncated induce protective immunity mice challenged necrophorum. polypeptides, individually or combination, inactivated native culture supernatant homogenized adjuvant injected into on days 0 21. Blood samples collected measure serum anti-leukotoxin antibody titers 0, 21 42 day 42, experimentally All immunogenic, GAS polypeptide eliciting least response. Two (BSBSE SH) induced significant protection against infection. Protection was better than supernatant.The study demonstrated that carries epitopes experimental fusobacterial infection, thus providing further evidence importance as