Functional gap junctions facilitate melanoma antigen transfer and cross-presentation between human dendritic cells.
作者： Ariadna Mendoza-Naranjo , Pablo J. Saéz , C. Christian Johansson , Marcos Ramírez , Dinka Mandaković
DOI: 10.4049/JIMMUNOL.178.11.6949
关键词:
摘要: Previously, we found that human dendritic cells (hDCs) pulsed with a melanoma cell lysate (MCL) and stimulated TNF-alpha (MCL/TNF) acquire mature phenotype in vitro are able to trigger tumor-specific immune responses when they used immunotherapy patients. In this study, describe MCL/TNF induces gap junction (GJ)-mediated intercellular communications promotes Ag transfer between ex vivo produced hDCs from also exhibit increased expression of the GJ-related protein connexin 43, which contributes GJ plaque formation after stimulation. The addition inhibitors suppresses tumor hDCs, thus reducing melanoma-specific T activation. summary, demonstrate MCL/TNF-stimulated can establish functional channels participate transfer, facilitating cross-presentation an effective cell-mediated response. These results suggest GJs formed cancer vaccination protocols could be essentials for establishment more efficient antitumor
jimmunol.org参考文章(32)
Marc Chanson, Jean-Paul Derouette, Isabelle Roth, Bernard Foglia, Isabelle Scerri, Tecla Dudez, Brenda R. Kwak, Gap junctional communication in tissue inflammation and repair Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1711, pp. 197- 207 ,(2005) , 10.1016/J.BBAMEM.2004.10.005
P. I. Jara, M. P. Boric, J. C. Saez, Leukocytes express connexin 43 after activation with lipopolysaccharide and appear to form gap junctions with endothelial cells after ischemia-reperfusion. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 7011- 7015 ,(1995) , 10.1073/PNAS.92.15.7011
Martin Rosendaal, Chris Jopling, Hematopoietic capacity of connexin43 wild-type and knock-out fetal liver cells not different on wild-type stroma. Blood. ,vol. 101, pp. 2996- 2998 ,(2003) , 10.1182/BLOOD-2002-07-2028
Xiaojun Guan, Susan Wilson, Keith K. Schlender, Randall J. Ruch, Gap-junction disassembly and connexin 43 dephosphorylation induced by 18β-glycyrrhetinic acid Molecular Carcinogenesis. ,vol. 16, pp. 157- 164 ,(1996) , 10.1002/(SICI)1098-2744(199607)16:3<157::AID-MC6>3.0.CO;2-E
CH.U. BRAND, TH. HUNZIKER, TH. SCHAFFNER, A. LIMAT, H.A GERBER, L.R BRAATHEN, Activated immunocompetent cells in human skin lymph derived from irritant contact dermatitis: an immunomorphological study. British Journal of Dermatology. ,vol. 132, pp. 39- 45 ,(1995) , 10.1111/J.1365-2133.1995.TB08622.X
Frank O. Nestle, Selma Alijagic, Michel Gilliet, Yuansheng Sun, Stephan Grabbe, Reinhard Dummer, Günter Burg, Dirk Schadendorf, Vaccination of melanoma patients with peptide- or tumor lysate-pulsed dendritic cells. Nature Medicine. ,vol. 4, pp. 328- 332 ,(1998) , 10.1038/NM0398-328
Joost Neijssen, Carla Herberts, Jan Wouter Drijfhout, Eric Reits, Lennert Janssen, Jacques Neefjes, Cross-presentation by intercellular peptide transfer through gap junctions Nature. ,vol. 434, pp. 83- 88 ,(2005) , 10.1038/NATURE03290
E. Oviedo-orta, T. Hoy, W. H. Evans, Intercellular communication in the immune system: differential expression of connexin40 and 43, and perturbation of gap junction channel functions in peripheral blood and tonsil human lymphocyte subpopulations Immunology. ,vol. 99, pp. 578- 590 ,(2000) , 10.1046/J.1365-2567.2000.00991.X
L Zitvogel, J I Mayordomo, T Tjandrawan, A B DeLeo, M R Clarke, M T Lotze, W J Storkus, Therapy of murine tumors with tumor peptide-pulsed dendritic cells: dependence on T cells, B7 costimulation, and T helper cell 1-associated cytokines. Journal of Experimental Medicine. ,vol. 183, pp. 87- 97 ,(1996) , 10.1084/JEM.183.1.87
Beatrice Thurner, Ina Haendle, Claudia Röder, Detlef Dieckmann, Petra Keikavoussi, Helmut Jonuleit, Armin Bender, Christian Maczek, Doris Schreiner, Peter von den Driesch, Eva B. Bröcker, Ralph M. Steinman, Alexander Enk, Eckhart Kämpgen, Gerold Schuler, Vaccination with Mage-3a1 Peptide–Pulsed Mature, Monocyte-Derived Dendritic Cells Expands Specific Cytotoxic T Cells and Induces Regression of Some Metastases in Advanced Stage IV Melanoma Journal of Experimental Medicine. ,vol. 190, pp. 1669- 1678 ,(1999) , 10.1084/JEM.190.11.1669