Screening and validation of reference genes for qPCR analysis in gonads and embryos of Takifugu bimaculatus
作者： Zhaowei Zhong , Lulu Ao , Liping Zhao , Zeyu Zhang , Yonghua Jiang
DOI: 10.1016/J.AAF.2020.10.002
关键词:
摘要: Abstract Suitable reference genes are one of the necessary conditions for obtaining reliable results by real-time fluorescence quantitative PCR (qPCR). In this study, expression 10 common candidate (18s rRNA, rps27, cnbp, rpl7, ube2, hsp-at, gapdh, β-actin, rpl13a, 1-ef1a) at different developmental stages gonad and embryo Takifugu bimaculatus were analyzed qPCR. And stability these is GeNorm, NormFinder Bestkeeper softwares. The showed that 1-ef1a was highest (p
sci-hub.st 参考文章(41)
Megan Louise Barney, Molecular investigations on sex determination and differentiation pathways in the common carp, Cyprinus carpio ,(2010)
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
A. Y. Wen, F. You, P. Sun, J. Li, D. D. Xu, Z. H. Wu, D. Y. Ma, P. J. Zhang, CpG methylation of dmrt1 and cyp19a promoters in relation to their sexual dimorphic expression in the Japanese flounder Paralichthys olivaceus Journal of Fish Biology. ,vol. 84, pp. 193- 205 ,(2014) , 10.1111/JFB.12277
M.C. Stride, A. Polkinghorne, B.F. Nowak, Chlamydial infections of fish: diverse pathogens and emerging causes of disease in aquaculture species Veterinary Microbiology. ,vol. 170, pp. 19- 27 ,(2014) , 10.1016/J.VETMIC.2014.01.022
Philipp Olias, Iris Adam, Anne Meyer, Constance Scharff, Achim D. Gruber, Reference Genes for Quantitative Gene Expression Studies in Multiple Avian Species PLoS ONE. ,vol. 9, pp. e99678- ,(2014) , 10.1371/JOURNAL.PONE.0099678
Bao-cun Zhang, Li Sun, Zhi-zhong Xiao, Yong-hua Hu, Quantitative real time RT-PCR study of pathogen-induced gene expression in rock bream (Oplegnathus fasciatus): Internal controls for data normalization Marine Genomics. ,vol. 15, pp. 75- 84 ,(2014) , 10.1016/J.MARGEN.2014.03.001
Chang Geng Yang, Xian Li Wang, Juan Tian, Wei Liu, Fan Wu, Ming Jiang, Hua Wen, Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus). Gene. ,vol. 527, pp. 183- 192 ,(2013) , 10.1016/J.GENE.2013.06.013
Huifen Liu, Xingjiang Mu, Lang Gui, Maoliang Su, Hong Li, Guang Zhang, Zhenhao Liu, Junbin Zhang, Characterization and gonadal expression of FOXL2 relative to Cyp19a genes in spotted scat Scatophagus argus. Gene. ,vol. 561, pp. 6- 14 ,(2015) , 10.1016/J.GENE.2014.12.060
Ming-Hui Li, Hui-Hui Yang, Meng-Ru Li, Yun-Lv Sun, Xiao-Long Jiang, Qing-Ping Xie, Ting-Ru Wang, Hong-Juan Shi, Li-Na Sun, Lin-Yan Zhou, De-Shou Wang, Antagonistic roles of Dmrt1 and Foxl2 in sex differentiation via estrogen production in tilapia as demonstrated by TALENs. Endocrinology. ,vol. 154, pp. 4814- 4825 ,(2013) , 10.1210/EN.2013-1451
Maurício Rocha-Martins, Brian Njaine, Mariana S. Silveira, Avoiding Pitfalls of Internal Controls: Validation of Reference Genes for Analysis by qRT-PCR and Western Blot throughout Rat Retinal Development PLoS ONE. ,vol. 7, pp. e43028- ,(2012) , 10.1371/JOURNAL.PONE.0043028