1H NMR Metabolic Fingerprinting to Probe Temporal Postharvest Changes on Qualitative Attributes and Phytochemical Profile of Sweet Cherry Fruit

摘要: Sweet cherry fruits (Prunus avium cvs. ‘Canada Giant’, ‘Ferrovia’) were harvested at commercial maturity stage and analyzed harvest after maintenance room temperature (storage ~ 20°C, shelf life) for 1, 2, 4, 6 8 days, respectively. Fruit initially respiration rate, qualitative attributes textural properties: Giant’ fruit characterized by higher weight losses stem browning index, being more intense over the late stages of life period; meanwhile ‘Ferrovia’ possessed appreciably better performance even extended period. A gradual decrease rate was monitored in both cultivars, culminated days 20°C. The sweet nutraceutical profile using an array instrumental techniques (spectrophotometric assays, HPLC, 1H-NMR). antioxidant capacity enhanced with progress period, concomitant increased levels total anthocyanin phenolic compounds. presented contents neochlorogenic acid p-coumarolquinic throughout We further developed 1H-NMR method that allows study primary secondary metabolites a single running, without previous separation isolation procedures. Diagnostic peaks located aliphatic region sugars organic acids, aromatic compounds 8.2 to 8.6 ppm anthocyanins. This NMR-based methodology provides unifying tool quantitative characterization metabolite changes fruits; it is also expected be exploited monitoring temporal other fleshy fruits.