s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis
作者： Jin Li , Ross Berbeco , Robert J. Distel , Pasi A. Jänne , Lilin Wang
DOI: 10.1093/NAR/GKM403
关键词:
摘要: The rapidly growing understanding of human genetic pathways, including those that mediate cancer biology and drug response, leads to an increasing need for extensive reliable mutation screening on a population or single patient basis. Here we describe s-RT-MELT, novel technology enables highly expanded enzymatic scanning in samples germline low-level somatic mutations, SNP discovery. GC-clamp-containing PCR products from interrogated wild-type are hybridized generate mismatches at the positions mutations over one multiple sequences in-parallel. Mismatches converted double-strand breaks using DNA endonuclease (Surveyor TM ) oligonucleotide tails enzymatically attached position mutations. A application selective amplification mutationcontaining fragments. Subsequently, melting curve analysis, conventional nano-technology real-time platforms, detects contain high-throughput closed-tube manner. We apply s-RT-MELT p53 EGFR cell lines clinical demonstrate its advantages rapid, multiplexed variation medicine.
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