Joint Profiling of miRNAs and mRNAs Reveals miRNA Mediated Gene Regulation in the Göttingen Minipig Obesity Model

摘要: Obesity and its comorbidities are an increasing challenge for both affected individuals health care systems, worldwide. In obese individuals, perturbation of expression protein-coding genes microRNAs (miRNA) seen in obesity-relevant tissues (i.e. adipose tissue, liver skeletal muscle). miRNAs small non-coding RNA molecules which have important regulatory roles a wide range biological processes, including obesity. Rodents widely used animal models human diseases However, not all research is applicable or diseases. contrast, pigs emerging as excellent model obesity studies, due to their similarities metabolism, digestive tract genetics, when compared humans. The Gottingen minipig sized easy-to-handle pig breed has been extensively modeling obesity, capacity develop severe fed ad libitum. aim this study was identify differentially expressed model. Liver, muscle abdominal tissue were sampled from 7 lean minipigs. Differential gene investigated using high-throughput quantitative real-time PCR (qPCR) on 90 mRNAs 72 miRNAs. results revealed de-regulation several inflammation-relevant examined. Many that known be de-regulated humans confirmed the minipigs these target sites opposing direction gene, confirming miRNA-mediated regulation These confirm translational value studies.