Overexpression of Drosophila juvenile hormone esterase binding protein results in anti-JH effects and reduced pheromone abundance.

摘要: The titer of juvenile hormone (JH), which has wide ranging physiological effects in insects, is regulated part by JH esterase (JHE). We show that overexpression Drosophila melanogaster the JHE binding protein, DmP29 results a series apparent anti-JH effects. hypothesize functions transport such over- or under-expression increased decreased degradation at specific sites respectively. Overexpression during first second instar was lethal, while third resulted eclosion small adults. newly eclosed flies reduced ovarian development and fecundity addition to reducing abundance aggregation pheromone (cis-vaccenyl acetate) males courtship (cis,cis-7,11-heptacosadiene) females. Both sexes also had lower levels 23 25 carbon monoenes. Females exhibited receptivity mating, male-male behavior, with both being hyperactive: Male covered 2.7 times distance control 2.9 maximum velocity. Application analog methoprene reversed impaired development, supporting role for production this phenotype. Rather than increasing lifespan as expected from deficiency, life span adult may result hyperactivity these flies. Underexpression longevity, titers pupal JHE. An alternative hypothesis, mitochondrial dysfunction rather JH-mediated phenotypes, discussed.