Crystal Structure of the DNA Binding Domain of the Replication Initiation Protein E1 from Papillomavirus

摘要: Papillomaviral infection causes both benign and malignant lesions is a necessary cause of cervical carcinoma. Replication this virus requires the replication initiation proteins E1 E2, which bind cooperatively at origin (ori) as an (E1)2-(E2)2-DNA complex. This precursor to larger complexes that distort unwind ori. We present crystal structure DNA binding domain refined 1.9 A resolution. Residues critical for are located on extended loop alpha helix. identify dimerization surface by selective mutations E1/E1 interface observed in propose model (E1)2-DNA These other observations suggest how orchestrates assembly hexameric helicase